Coding

Part:BBa_K109700:Design

Designed by: Ingrid Swanson   Group: iGEM08_University_of_Washington   (2008-07-28)


lac permease in yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 881
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Changed the stop codon from TGA to TAATAA to comform to BioBricks assembly standards.


Source

This part is from PCR amplification from K. lactis genomic DNA stored in an E. coli K. lactis ORF library.

References